Xenbase Image ID: 180384

Figure 5 | Znrf3 is coexpressed- and cooperates with Ptprk in early Xenopus embryos (A) Spatial expression of znrf3 in Xenopus tropicalis embryos at blastula (animal view), gastrula (hemisected dorsal to the right), neurula, tailbud and tadpole stages. ba, branchial arches; bp, blastopore; hb, hindbrain; mb, midbrain; nt, neural tube; ov, ovic vesicle; SO Spemann organizer. (B) Representative phenotypes of tailbud stage Xenopus tropicalis embryos injected animally at 2- to 8- cell stage as indicated. (C) Quantification of phenotypes shown in (B). The number of embryos per condition is indicated on the top. (D) Whole mount in situ hybridization of gsc and chordin in gastrula embryos (stage 10.5). Embryos were injected at 2- to 8-cell stage animally with Co or ptprk Mo with or without ZNRF3 RNA. For quantification, see Figure 5 – figure supplement 1C. (E) Topflash reporter assay performed with neurulae (stage 18). Embryos were injected animally at 2- to 8-cell stage as indicated. Suboptimal dosages of ptprk or znrf3 Mos were used in this experiment. Normalized Topflash activity of Co Mo injected embryos was set to 1. (F) Topflash reporter assay performed with neurulae (stage 18). Embryos were injected animally at 2- to 8-cell stage as indicated. Normalized Topflash activity of Co Mo injected embryos was set to 1. Data in all graphs are displayed as means ± SD, and show one representative of multiple independent experiments with three biological replicates. RLU, relative light units. ** P < 0.01. Image published in: Chang LS et al. (2020) © 2020, Chang et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue znrf3 X. tropicalis Throughout NF stage 2 (2-cell) animal hemisphere znrf3 X. tropicalis Throughout NF stage 10.5 upper blastopore lip dorsal marginal zone znrf3 X. tropicalis Throughout NF stage 13 circumblastoporal collar znrf3 X. tropicalis Throughout NF stage 23 hindbrain midbrain neural tube znrf3 X. tropicalis Throughout NF stage 35 and 36 hindbrain midbrain otic vesicle pharyngeal region Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr WT + ptprk MO + tailbud (whole-mount microscopy) Fig. 5B, row2, col1 Anatomical Phenotype abnormal anterior/posterior axis specification decreased length of anterior-posterior axis decreased size of the head Xtr WT + znrf3 MO + tailbud (whole-mount microscopy) Fig. 5B, row2, col2, Fig. 5C Anatomical Phenotype abnormal anterior/posterior axis specification decreased length of anterior-posterior axis decreased size of the head Xtr WT + znrf3 MO + tailbud (whole-mount microscopy) Fig. 5B, row3, col1, Fig. 5C Anatomical Phenotype abnormal anterior/posterior axis specification decreased length of anterior-posterior axis decreased size of the head Xtr WT + ptprk MO + NF 10.5 (in situ hybridization) Fig. 5D, row1, col2 Expression Phenotype decreased amount gsc expression in dorsal marginal zone decreased amount gsc expression in upper blastopore lip Xtr Wt + Hsa.ZNRF3 + NF 10.5 (in situ hybridization) Fig. 5D, row1, col4 Expression Phenotype increased amount gsc expression in dorsal marginal zone increased amount gsc expression in upper blastopore lip Xtr WT + ptprk MO + NF 10.5 (in situ hybridization) Fig. 5D, row2, col2 Expression Phenotype decreased amount chrd.1 expression in dorsal marginal zone decreased amount chrd.1 expression in upper blastopore lip Xtr Wt + Hsa.ZNRF3 + NF 10.5 (in situ hybridization) Fig. 5D, row2, col4 Expression Phenotype increased amount chrd.1 expression in dorsal marginal zone increased amount chrd.1 expression in upper blastopore lip Larger Image Printer Friendly View

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