Xenbase Image ID: 1808

Fig. 2. Over-expression of Xhes6 increases XmyoD expression in vivo. (A) Constructs used. Xhes6 constructs were myc tagged at the N-terminus. In the DBM mutant, the basic amino acids in the DNA domain were mutated to acidic residues (underlined) whilst in the δWRPW mutant, the C-terminal WRPW protein–protein interaction motif was replaced by a stop codon (red triangle). (B) Western blotting with an anti-myc antibody to detect Xhes6 protein (upper panel) and an anti-α-tubulin antibody loading control (lower panel). (C–L) Effects of overexpression of wild-type and mutant Xhes6 500 pg of Xhes6, DBM or δWRPW mRNA was injected into marginal zone of a blastomere at 2-cell stage, along with β-galactosidase mRNA (red staining) and analyzed at gastrula stage for expression of XmyoD (C–F), Xbra (G, H), Xwnt8 (I, J) and Chordin (K, L) by whole-mount in situ hybridisation. The side view of the area within the yellow box is shown on the right of each panel. Image published in: Murai K et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Throughout NF stage 10.25 mesoderm chrd chd, chordin, chrd.1, LOC108716939, X-chordin Xenopus Throughout NF stage 10.25 mesoderm wnt8a wnt-8, wnt8, Xwnt-8, Xwnt8 Xenopus Throughout NF stage 10.25 mesoderm myod1 mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD Xenopus Throughout NF stage 10.25 mesoderm Image source: Published Larger Image Printer Friendly View

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