Xenbase Image ID: 181081

Fig. 3. Transgenic analysis of the effects of overexpressing myc-ID dimer on animal development. (a) Schematic representation of the construct used for transgenesis. The heat shock-inducible promoter drives the expression of the myc-ID dimer transgene. The construct also harbors GFP driven by γ-crystallin promoter as a marker to identify transgenic animals. (b) A transgenic (Tg) and wild type (WT) Xenopus laevis tadpole at stage 46. The presence of GFP in the eye (arrows) indicates the presence of the transgene, myc-ID dimer, in the Tg tadpole. The arrowheads indicate the auto-fluorescence, likely due to the yolk remaining in the tadpoles. (c) Experimental scheme using myc-ID dimer transgenic tadpoles. WT and Tg tadpoles at stage 46 (about 10 days old, shortly after feeding begins at stage 45) were heat shocked for 1 h at 33–34 °C on days 1–30. Some of the tadpoles were also treated with 1 nM of T3 for the first 5 days. The developmental stages of the tadpoles were examined every 5 days. Note that the experiment ended after 30 days, when the tadpoles reached stage 55 and plasma thyroid hormones (T3, 3,5,3′-triiodo-l-thyronine, and T4, 3,5,3′,5′-tetraiodo-l-thyronine) become detectable, which would lead to the dissociation of both dnN-CoR and endogenous corepressors from TR. Image published in: Sato Y et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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