Xenbase Image ID: 181082

Fig. 4. Heat shock induces the expression of the myc-ID dimer transgene. (a) The expression of the myc-ID dimer mRNA is induced in the transgenic tadpoles after heat shock. Transgenic tadpoles were identified by the GFP in the eyes at the end of the treatments. Total RNAs were isolated from individual wild type (WT) and transgenic (Tg) animals before (0) and after 1, 3, 5, 10 days of heat shock with or without T3 treatment. The cDNAs reverse-transcribed from the total RNAs were subjected to PCR using primers specific for the myc-ID dimer or ribosomal protein L8 (rpL8) (Shi and Liang, 1994) as an internal control. Shown here is representative of three independent experiments with similar results. Each band corresponds to one WT or Tg animal heat-shocked for indicated number of days. (b) Immunoprecipitation (IP) analysis confirms the expression of myc-ID dimer protein in the transgenic animals after heat shock treatment. The protein lysates from WT and Tg animals after 30 days of heat shock were immunoprecipitated with anti-myc antibody. IP samples were immunoblotted with anti-myc antibody (upper panel). Pre-IP samples were blotted with β-actin antibody (lower panel) as a loading control. Image published in: Sato Y et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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