Xenbase Image ID: 1835

Fig. 6. Xisl-1 is required for cardiac marker gene expression. 10 ng islMO were injected unilaterally into the dorsal marginal zone of 8-cell stage embryos. At st. 28 these embryos were analysed for cardiac gene expression by whole mount in situ hybridization. Xisl-1 loss-of-function results in a reduction or even loss of expression of the early cardiac gene Tbx20, as well as of the differentiation marker genes Troponin Ic (TnIc) and cardiac-α-actin on the injected side (arrows). The specificity of the morpholino effect was verified by coinjection of RNA transcribed from the δisl-1 deletion construct, whose translation is not inhibited by the islMO. Coinjection of δisl-1 RNA and islMO partially restored the expression of the investigated marker genes. Image published in: Brade T et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbx20.L XTBX20 X. laevis Throughout NF stage 28 heart cement gland primordium heart primordium tnni3.L c-troponin, cardiac tropin T, cardiac troponin I, cTnI, ctnIc, cTNT, TIc, TnIc, tnnic, troponin-ic, XcTnI, XTnIc X. laevis Throughout NF stage 28 heart myocardium heart primordium actc1.L actc, actna1, cardiac actin, cardiac alpha actin, muscle actin X. laevis Throughout NF stage 28 heart heart primordium Image source: Published Larger Image Printer Friendly View

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