XB-IMG-1850
Xenbase Image ID: 1850
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Fig. 1. A screen for differentially expressed genes in a mucociliary epithelium. (A) The Xenopus epidermis is a salt-and-pepper mix of mucus-secreting goblet cells and ciliated cells. Ciliated cells are marked by red α-tubulin staining. Small secretory cells are labeled with an asterisk. All other cells are goblet cells. (B) At higher magnification, membrane-GFP (green) reveals numerous exocytic vesicles at the apical surface of goblet cells, and α-tubulin staining (red) reveals cilia. (C) The vesicles of small secretory cells are shown by phalloidin stain (green) and a neighboring ciliated cell is marked by alpha-tubulin stain (red). (c′) A high magnification view of a small secretory cell shows visible vesicles. (D) A diagram of cell types in the Xenopus epidermis. Goblet cells are the predominant cell type. Ciliated and small secretory cells (asterisks) are scattered throughout. (E) In situ hybridization for genes expressed in ciliated cells produces regularly spaced dark spots on a light background. (F) Genes expressed in goblet cells produce a reciprocal pattern. (G) Small secretory cells are visible as unevenly scattered dark spots. The full set of differentially expressed genes can be found in Supplemental Table 1. Image published in: Hayes JM et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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