Xenbase Image ID: 1957

Fig. 2. Expression patterns of Xpreproapelin and Xmsr mRNA. (A) RT-PCR analysis of Xpreproapelin and Xmsr mRNA during development. Ornithine decarboxylase (ODC) was used as an internal control. (B, C) Expression of Xpreproapelin mRNA at stage 10. Xpreproapelin mRNA was detected in the marginal zone, at the bilateral area of the dorsal lip (arrowheads). Inset in panel B shows a magnified view of the expression. (D, E) Expression of Xpreproapelin mRNA at stage 18, in the anterior ventral region of the embryo (arrowheads). (F, G) Expression of Xmsr mRNA at stage 10, in the marginal zone except for dorsal marginal zone (arrowheads). (H, I) Expression of Xmsr mRNA at stage 18, in the anterior ventral region of the embryo (arrowheads). (J–L) Expression of Xpreproapelin mRNA at stage 22, in regions lateral and posterior to the cement gland (CG), the dorsal posterior part of the embryo, and the dorsal midline. (M–O) Expression of Xmsr mRNA at stage 22, in areas lateral and posterior to the cement gland (CG), the dorsal posterior part of the embryo, and the dorsal midline. (P–R) Expression of Xpreproapelin mRNA at stage 30, around the anterior and posterior cardinal vein (ACV white arrowhead, PCV black arrowhead), aortic arcs (AA, arrowhead), dorsal longitudinal anastomosing vessel (DLAV, arrowhead), notochord (NC), neural tube (NT), and tailbud. (S–U) Expression of Xmsr mRNA at stage 30, in the anterior and posterior cardinal vein (ACV white arrowhead, PCV black arrowheads), aortic arcs (AA, arrowheads), endocardium (EC arrowhead), vascular vitelline network (VVN arrowhead), and tailbud. B, F, L, O: dorsal view;, C, G: vegetal view, dorsal upward, D H; anterior view, E, I, K, N, R, U: ventral view, J, M, P, Q, S, T: lateral view. Panels Q and T are magnified views of the dorsal part of the cleared embryos shown in panels P and S, respectively. Image published in: Inui M et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Throughout NF stage 10 marginal zone dorso-lateral marginal zone ventro-lateral marginal zone ventral marginal zone aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Throughout NF stage 18 anterior ventral aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Throughout NF stage 22 anterior ventral neural tube aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Throughout NF stage 29 and 30 posterior cardinal vein tail bud anterior cardinal vein head mesoderm endocardium notochord dorsal longitudinal anastomosing vessel aortic arch neural tube apln.L apelin, apln-a, apln-b, preproapelin, Xapelin X. laevis Throughout NF stage 10 dorso-lateral marginal zone apln.L apelin, apln-a, apln-b, preproapelin, Xapelin X. laevis Throughout NF stage 18 anterior ventral apln.L apelin, apln-a, apln-b, preproapelin, Xapelin X. laevis Throughout NF stage 22 neural tube tail bud anterior ventral apln.L apelin, apln-a, apln-b, preproapelin, Xapelin X. laevis Throughout NF stage 29 and 30 posterior cardinal vein tail bud anterior cardinal vein head mesoderm dorsal longitudinal anastomosing vessel aortic arch Image source: Published Larger Image Printer Friendly View

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