Xenbase Image ID: 2089

Fig. 1. Inhibition of SHP-2 activity results in loss of MHC expression. Tissue explants show identical cardiac expression profiles as intact Xenopus embryos. (A) Whole-mount antibody staining of cardiac differentiation with tropomyosin (Tmy; red) or myosin heavy chain (MHC; red) antibodies as indicated, in whole embryos and cardiac explants at stages 22 (neurula), 29 (tailbud) and 37 (tadpole). (B) Whole-mount in situ hybridization for early heart markers Tbx5, Tbx20, Nkx2.5 in whole embryos and cardiac explants at stage 22, 29 and 37, as indicated. (C) MHC expression is dependent on SHP-2 activity. Whole-mount antibody staining for MHC (red) in cardiac explants taken from uninjected (control) embryos or embryos injected with Shp-2 N308D and treated with either buffer or DMSO carrier as controls or with NSC-87877 as indicated. BF, bright field. Image published in: Langdon YG et al. (2007) Copyright © 2007. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tpm1.L alpha-tm, alpha-tropomyosin, CH1 X. laevis Sometime during NF stage 22 to NF stage 37 and 38 heart somite myh6.S aMHC, ck231, MHC, MHC-alpha, MHCa, MHCalpha, muz, muzak, XCMHC X. laevis Sometime during NF stage 22 to NF stage 37 and 38 heart nkx2-5.S AR2, csx, Nkx-2.5, nkx-2.5, nkx2-5-a, nkx2-5-b, nkx2.5, tinman, XNkx-2.5, XNkx2-5 X. laevis Sometime during NF stage 22 to NF stage 37 and 38 heart tbx20.L XTBX20 X. laevis Sometime during NF stage 22 to NF stage 37 and 38 heart tbx5.L tbx-5, tbx5-a, tbx5-b, xtbx-5, xtbx5 X. laevis Sometime during NF stage 22 to NF stage 37 and 38 heart Image source: Published Larger Image Printer Friendly View

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