Xenbase Image ID: 22412

Fig. 3. Analysis of xFog expression pattern by RNA in situ hybridization on developmentally staged (albino) embryos. The pattern of xFog expression (A-E) in developing Xenopus embryos is a combination of the xGata gene expression patterns (F-I). (A) At stage 15, expression starts in the presumptive heart and the presumptive ventral blood island, possibly extending up into the presumptive pronephric region, but excluded from the presumptive liver. P, posterior. (B) At stage 23, expression is pronounced in heart primordia, similar to xGata-6 (F). (C) At stage 27, expression is high in heart tube, and in the ventral blood island (indicated by stars, very similar to xGata-2, shown in G). Expression is also seen in midbrain and hindbrain, and in the otic vesicle (indicated by white arrow, similar to xGata-3 shown in H).(D) At stage 33, xFog continues to be highly expressed in the heart and ventral blood island. The faint staining in the region around the midline is either the pronephros, similar to xGata-3 (H) or a blood vessel. Expression is also seen in the eyes and brain (similar to xGata-3 (E,J) At stage 38, expression of xFog is pronounced in heart and surrounding tissue (similar to xGata-4 in I), midbrain and the posterior region of the ventral blood island. (F) xGata-6, stage 23; (G) xGata-2, stage 28; (H) xGata-3, stage 26; (I) xGata-4, stages 38 (bottom) and 40 (top); (J) xFog, stage 38, cleared embryo. (K,L) Transverse section of a stage 29 embryo. xFog is expressed in the mesodermal layer of the ventral blood island (original magnification: (K) ´100, (L) ´400; v, ventral; d, dorsal; se, sensorial ectoderm; en, endodermal yolk mass; m, mesodermal layer. Image published in: Deconinck AE et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 27 heart ventral blood island ventral gata3.S GATA-3, XGATA-3, XGATA3 X. laevis Throughout NF stage 33 and 34 brain eye pronephric duct zfpm1.L fog, fog-1, fog1, friend of GATA, LOC108714099, Xfog X. laevis Throughout NF stage 15 cardiac mesoderm gata6.S GATA-6, gata6-a, gata6-b, gata6a, gata6b, tGata6, XGATA-6, XGATA6 X. laevis Throughout NF stage 23 cardiac mesoderm heart primordium zfpm1.L fog, fog-1, fog1, friend of GATA, LOC108714099, Xfog X. laevis Throughout NF stage 23 cardiac mesoderm heart primordium zfpm1.L fog, fog-1, fog1, friend of GATA, LOC108714099, Xfog X. laevis Throughout NF stage 27 brain heart otic vesicle ventral ventral blood island midbrain hindbrain zfpm1.L fog, fog-1, fog1, friend of GATA, LOC108714099, Xfog X. laevis Sometime during NF stage 33 and 34 to NF stage 37 and 38 brain heart eye ventral ventral blood island pronephric duct midbrain zfpm1.L fog, fog-1, fog1, friend of GATA, LOC108714099, Xfog X. laevis Throughout NF stage 29 and 30 endoderm cardiac mesoderm sensorial layer of neurectoderm vegetal yolk mass gata4.L gata-4, gata4-a, gata4-b, xGATA-4, xgata4 X. laevis Throughout NF stage 37 and 38 to NF stage 40 cardiac mesoderm head region Image source: Published Larger Image Printer Friendly View

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