Xenbase Image ID: 2262

Fig. 2. Validation of endoderm-enriched transcripts. (A) At stage 11, RNA was isolated from animal cap ectoderm (An), endoderm-enriched vegetal tissue (Veg) and mesendoderm enriched equatorial tissue (Eq), and assayed by RT-PCR to validate the expression profile of endoderm-enriched transcripts. The black histogram shows relative normalized expression from the array and the grey histograms show the relative expression levels in RT-PCR normalized to the loading control, ODC. Sox17α, Xbra and Epidermal keratin (Epi-K) are positive controls for the dissections. Fifty-one out of 54 genes were confirmed to have vegetal expression that was three times greater than animal cap expression (see Table S4 in the supplementary material) and 12 representative genes are shown [Xl.10408, Xl.13381, Xl.14891, Xl.15171, Xl.16410, Xl.16875 (Mixer-b), Xl.2410, Xl.2554, Xl.4709, Xl.4935, Xl.5999, Xl.8924 (gap junction subunit 6)]. (B) In situ hybridization to bisected gastrula with probes to the indicated genes validates their endoderm-enriched expression. Xl.1191 and Xl.15054 are not detected until neurula stage (st15). Image published in: Sinner D et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue mixer tMixer Xenopus Throughout NF stage 11 endoderm cxcr4.S cd184, cxcr4-a, cxcr4-b, fb22, hm89, hsy3rr, lap3, lcr1, lestr, npy3r, npyr, npyrl, npyy3r, xcxcr4 X. laevis Throughout NF stage 11 involuted dorsal mesoderm involuted ventral mesoderm endoderm vegetal yolk mass wnt11.S Wnt11-R, WNT11-related, wnt11a, Wnt11r X. laevis Throughout NF stage 11 endoderm dorsal vegetal yolk mass fbln1.L X. laevis Throughout NF stage 15 endoderm vegetal yolk mass epn1.S X. laevis Throughout NF stage 11 involuted dorsal mesoderm involuted ventral mesoderm pnhd.L pinhead X. laevis Throughout NF stage 11 involuted ventral mesoderm upper blastopore lip foxa4.L fkh1, foxa4-a, foxa4-b, FoxA4a, pintallavis, XFD-1, xfkh1, XFKHI, xkfh-1 X. laevis Throughout NF stage 11 involuted dorsal mesoderm involuted ventral mesoderm arhgap4.S X. laevis Throughout NF stage 11 endoderm involuted dorsal mesoderm involuted ventral mesoderm slc12a3l.L LOC108695638, ncct, scl12a3l, slc12a3-like, slc12a3.2 X. laevis Throughout NF stage 11 endoderm endomesoderm vegetal yolk mass gjb1.L cmtx, cmtx1, connexin 30, connexin 32, connexin-30, connexin32, cx30, cx32 X. laevis Throughout NF stage 15 endoderm endomesoderm vegetal yolk mass rnf38.L X. laevis Throughout NF stage 11 endomesoderm deep endoderm vegetal part, early involuting vegetal endoderm tmem150b.L tmem150b-a, tmem150b-b, tmem224 X. laevis Throughout NF stage 11 endomesoderm deep endoderm grip2.L LOC108715865, Xgrip2, Xgrip2.1 X. laevis Throughout NF stage 11 endoderm vegetal yolk mass pisdl.L pisd, pisd-like.2 X. laevis Throughout NF stage 11   Image source: Published Larger Image Printer Friendly View

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