Xenbase Image ID: 23200

Fig. 1. Effects of Hh signaling overexpression on eye DV polarity. (A,B) The red or light-blue β-gal staining identifies the injected side. The broken yellow circles highlight the eye region. (A) Unilateral injection of low doses of bhh mRNA (1 pg) upregulates Vax2 in the DR and reduces ET in stage 33 Xenopus embryos. Pax2, Vax1b and Raldh3 are partially upregulated, but not in the DR, and the Pax6 domain is slightly reduced in these embryos. On the uninjected side, Vax2 is normally expressed in the OS and the VR; ET is expressed in the DR; Pax2, Vax1b and Raldh3 are expressed in the OS region and Pax6 is expressed in the retina region. (B) High doses of bhh mRNA (250 pg) upregulate Vax2, Pax2, Vax1b and Raldh3 throughout the eye, and repress ET and Pax6 expression. (C) Schematic representation of the results shown in A and B. Low Hh levels partially expand the OS and ventralize in part the DR. Purple indicates the overlap of ventral and dorsal character in the most dorsal retina. High Hh levels transform the entire retina into OS. Image published in: Lupo G et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue vax1 vax, vax1-a, vax1-b, vax1b, vax1b-a, xvax1, xvax1b Xenopus Throughout NF stage 33 and 34 optic stalk retina ventral pax6 an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 Xenopus Throughout NF stage 33 and 34 retina lens diencephalon pax2 LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 Xenopus Throughout NF stage 33 and 34 optic stalk retina ventral vax2 dres93, vax2-a, vax2-b, vax3, xvax2 Xenopus Throughout NF stage 33 and 34 optic stalk retina ventral aldh1a3 raldh3 Xenopus Throughout NF stage 33 and 34 optic stalk retina ventral Image source: Published Larger Image Printer Friendly View

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