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Fig. 7. Organiser gene expression in FGFR-deficient embryos. (A-H) Four-cell embryos were treated with 80 μM SU5402 until early gastrula stage 10.5 and fixed for in situ hybridisation. This treatment yielded class V embryos. In A-C, embryos were cut in two halves along the dorsoventral midline prior to hybridisation in order to improve probe penetration. The organiser genes cerberus (cerb; A), dickkopf1 (dkk1, B), hex (C), otx2 (D), goosecoid (gsc; E) and chordin (chd; F) are normally expressed in class V embryos, whereas noggin (nog; G) is not. Conversely, the epidermis inducer bmp4 is ectopically expressed in the dorsal ectoderm of class V embryos (H). Thus, BMP signalling is probably upregulated in prospective neural tissue in absence of FGF activity. (A-C) Dorsal towards the right. (D-G) Vegetal view, dorsal towards the top. (H) Dorsal view, anterior towards the top, broken lines indicate the blastopore. Image published in: Delaune E et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue cer1.S cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 X. laevis Throughout NF stage 10.5 dorsal marginal zone involuted dorsal mesoderm dkk1.S dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Throughout NF stage 10.5 dorsal marginal zone involuted dorsal mesoderm hhex.L hex, tHex, XHex X. laevis Throughout NF stage 10.5 dorsal marginal zone involuted dorsal mesoderm otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 10.5 dorsal marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.5 dorsal marginal zone chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10.5 dorsal marginal zone bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 10.5 ectoderm dorsal epidermis ventro-lateral marginal zone nog.L nog-A, nog1, noggin, noggin 1, noggin-1, Noggin1 X. laevis Throughout NF stage 10.5 dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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