Xenbase Image ID: 23258

Fig. 2. XNIF encodes for a vegetally localized RNA in Xenopus oocytes. Whole-mount in-situ hybridization was carried out using albino oocytes of different stages. For a detailed analysis, sections of stained stage I and stage II oocytes were prepared. (A) In stage I oocytes, XNIF RNA is enriched in the mitochondrial cloud (examples marked by arrows). The cell nucleus is indicated by a dashed line in one oocyte. (B) In stage II oocytes, XNIF accumulates at the tip of the vegetal cortex (red arrow). In stage III-IV oocytes, XNIF RNA is associated with the vegetal cortex (black arrow). (C) Vegetal-cortical localization of XNIF in stage V/VI oocytes. (D) Section of whole-mount in-situ stained stage I oocyte. XNIF transcript is enriched in the mitochondrial cloud adjacent to the germinal vesicle. (E) Section of whole-mount in-situ stained stage II oocyte. XNIF is associated with reticular structures in a wedge-shaped region beneath the germinal vesicle. Scale bar:∼ 100 μm in (A,D,E) and 1 mm in (B,C). (F) XNIF expression in a stage 33 Xenopus albino embryo. Whole-mount in-situ hybridization staining with a XNIF specific antisense probe is shown. Image published in: Claussen M et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nef1f.L nif X. laevis Throughout oocyte stage I mitochondrial cloud nef1f.L nif X. laevis Throughout NF stage 33 and 34 somite tail somite presomitic mesoderm rostral presomitic mesoderm nef1f.L nif X. laevis Sometime during oocyte stage II to oocyte stage IV vegetal cortex nef1f.L nif X. laevis Sometime during oocyte stage IV to oocyte stage V vegetal cortex Image source: Published Larger Image Printer Friendly View

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