Xenbase Image ID: 23297

Fig. 4. The Notch target gene Hairy2A produces an expansion of the neural crest population. Eight-cell embryos were injected in two blastomeres with 1 ng Hairy2A mRNA, the arrowhead indicates the injected side, which was visualized by alkaline phosphatase-mediated FITC inmunodetection. (A) N-tubulin expression is clearly reduced. (B) Immunodetection of the somite antigen 12/101 analyzed at stage 25. Note that there is no difference in the staining between the injected and uninjected side. (C) The domain of Xslug expression is expanded on the injected side, whereas Bmp4 is dramatically repressed (D,E). (E) Corresponds to a higher magnification of D. (F) Xmsx1 expression is increased on the injected side. Each experiment was performed at least twice with a minimum of 35 embryos. The effect seen in each experiment was observed in at least 70% of embryos. Image published in: Glavic A et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tubb2b.S NBT, neural beta-tubulin, NST, N-Tub, n-tubulin, ntubulin, Xn-tubulin X. laevis Throughout NF stage 12.5 neural plate snai2.S slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 16 neural crest neuroectoderm msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Throughout NF stage 16 neural crest neuroectoderm bmp4.L bmp2b, bmp2b1, bmp-4, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 25 neural tube neural plate neuroectoderm eye primordium Image source: Published Larger Image Printer Friendly View

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