Xenbase Image ID: 23313

Fig. 5. Xrx1 regulates the expression of genes that control cell proliferation and differentiation, and does not work through the Notch-Delta pathway at early neurula. (A-C,G,H,M) Comparison of the expression of Xrx1 to that of Zic2 (A,B), Xhairy2 (C), Xhairy1 (G), p27Xic1 (H) and X-Notch-1 (M) in stage 13 embryos. (D-F,I-L,N) Xrx1-injected embryos analyzed at stage 14 (D-F,J,K,N) or stage 18 (I,L). The probes used and the respective staining are indicated, color-coded, on the bottom of each panel. (A-D,F-O) Frontal views, dorsal towards the top; (E,P,Q) dorsoanterior views. The injected side of the embryos (to the right of vertical bars representing the midline) is indicated (inj). Red staining in F,I,J,K,L,N-Q and turquoise staining in D,E represent expression of co-injected lacZ lineage tracer. (F,J,K) Black arrowheads indicate the lateroventral expression domain of Xhairy2 (F), Xhairy1 (J) and p27Xic1 (K) in the uninjected control side of the embryos. White arrowheads indicate the corresponding expression domain in the injected side, which is expanded in the case of Xhairy2 (F) and repressed in the case of Xhairy1 (J) and p27Xic1 (K). Black brackets indicate the anterior expression domains of Zic2 (D,E), cyclinD1 (I) and Xoptx2 (L) in the control uninjected side; white brackets indicate the corresponding enlarged domains in the injected side. (O) Stage 14 embryo injected with Notch-ICD showing no significant change in Xrx1 expression. (P,Q) Stage 16 embryos injected with X-Delta-1stu and lacZ (P) or co-injected with Xrx1, X-Delta-1stu and lacZ (Q). Xrx1 represses N-tubulin expression in the trigeminal ganglion but does not affect N-tubulin posterior expansion. Arrows indicate the increase in density of N-tubulin-positive cells within the posterior neurogenic stripes caused by the block of lateral inhibition. The black arrowhead indicates N-tubulin expression in the trigeminal ganglion of the uninjected side; the white arrowhead indicates the absence of this expression domain in the injected side. Image published in: Andreazzoli M et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue notch1 notch, notch-1, x-notch-1, xnotch, xnotch1, xotch Xenopus Throughout NF stage 13 neural plate zic2 hpe5, xzic2, zic2-a, zic2-b, Zinc finger protein of the cerebellum 2 Xenopus Throughout NF stage 13 neural plate anterior neural fold six6 hpe2, optic six gene 2, optx2, six3.2, Six6.1, six6.2, XOptx2, XSix3, xSix6, XSix6.1, xsix6.2 Xenopus Throughout NF stage 18 eye primordium hes4 H2, hairy2, hairy2a, hairy2b, hes4-a, hes4-b, hes4a, hes4b, Xhairy2, XHairy2a, Xhairy2b Xenopus Throughout NF stage 13 neural plate anterior neural fold notochord hes1 hairy1, hes-1, hes1-a, hes1-b, Xhairy1 Xenopus Throughout NF stage 13 neural plate trigeminal ganglion rax rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 13 neural plate cdknx p27Xic-1, p27XIC1, p28, Xic-1, Xic1 Xenopus Throughout NF stage 13 to NF stage 14 eye neural plate rax rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A Xenopus Throughout NF stage 13 neural plate tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 16 neural plate eye primordium neuroectoderm trigeminal ganglion ccnd1 ccnd1-a, ccnd1-b, cyclind1, parathyroid adenomatosis 1, prad1 Xenopus Throughout NF stage 13 neural plate eye primordium Image source: Published Larger Image Printer Friendly View

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