Xenbase Image ID: 23365

Fig. 2. Temporal and spatial expression patterns of XRnf12. (A) Northern analysis. The 3.5 kb transcript is expressed throughout early embryogenesis, while the 3.0 kb transcript disappears at stage 11. 18S rRNA stained with ethidium bromide served as a loading control. Numbers at the top are developmental stages. (B-D) Overlapping expression of Xlim-1, XRnf12 and Ldb1 in the gastrula mesoderm as revealed by whole-mount in situ hybridization of sagittally bisected gastrula embryos (stage 10.5). Animal side is to the top, dorsal to the right. Arrowhead, blastopore. (E-J) Co-expression of XRnf12 and Ldb1 in neurula and tailbud stage embryos. Dorsal is to the top, anterior to the left. Arrowhead indicates profundal-trigeminal placodal area; numbers in the bottom right of each panel are the developmental stages. (K-P) Co-expression of XRnf12 and Ldb1 as revealed by cross sections of stained tailbud embryos (stage 26). Upper, or left, panels are bright-field images; lower, or right, panels show DAPI staining. XRnf12 (EG,K-M) and Ldb1 (H-J,N-P) are similarly expressed throughout early embryogenesis. Turquoise staining inside of the embryos shown in the cross sections resulted from BCIP staining as NBT was depleted by strong staining in the epidermis. Both genes are expressed similarly in the neural tube along the AP axis. ba, branchial arches; dc, diencephalon; ey, eye; hb, hindbrain; hm, head mesenchyme; nc, neural crest; ov, otic vesicle; p, pronephros; pf, pronephric field; sc, spinal cord. Image published in: Hiratani I et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rlim rlim-a, rlim-b, rnf12, rnf12b, XRnf12 Xenopus Throughout NF stage 10.5 ectoderm mesoderm lhx1 lim-1, Lim1, lim1, Xlim-1, Xlim1 Xenopus Throughout NF stage 10.5 mesoderm dorsal ventral ldb1 clim2, nli, xldb1 Xenopus Throughout NF stage 10.5 ectoderm mesoderm rlim rlim-a, rlim-b, rnf12, rnf12b, XRnf12 Xenopus Throughout NF stage 17 trigeminal placode rlim rlim-a, rlim-b, rnf12, rnf12b, XRnf12 Xenopus Throughout NF stage 22 trigeminal placode rlim rlim-a, rlim-b, rnf12, rnf12b, XRnf12 Xenopus Throughout NF stage 28 branchial arch pronephric mesenchyme tail bud ldb1 clim2, nli, xldb1 Xenopus Sometime during NF stage 18 to NF stage 22 trigeminal placode pronephric mesenchyme rlim rlim-a, rlim-b, rnf12, rnf12b, XRnf12 Xenopus Throughout NF stage 26 neural tube epidermis neural crest head mesoderm ldb1 clim2, nli, xldb1 Xenopus Throughout NF stage 26 neural tube epidermis neural crest head mesoderm ldb1 clim2, nli, xldb1 Xenopus Throughout NF stage 29 and 30 pronephric kidney branchial arch tail bud Image source: Published Larger Image Printer Friendly View

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