Xenbase Image ID: 23371

Fig. 8. Effects of Ldb1 or XRnf12 overexpression on organizer gene expression. Embryos injected dorsally with the mRNAs indicated were scored for expression of various marker genes in the organizer as assayed by whole-mount in situ hybridization. Genes categorized in group 1 (A-E, gsc, chd, Xotx2, XPAPC and cer) are downregulated by overexpression of either Ldb1 or XRnf12, whereas genes categorized in group 2 (F,G, XFKH1 and Xnot) are downregulated only by XRnf12, and those in group 3 (H,I, dkk1 and Mix.1) are downregulated only by Ldb1. Xbra (J) is only slightly affected by Ldb1 overexpression. Numbers indicate the frequency of the phenotype observed: numbers in red indicates downregulation, whereas numbers in black or white indicates normal expression. Note that downregulation of gene expression by either Ldb1 or XRnf12 overexpression was restored upon co-expression of both, and that the rescuing effect of XRnf12 co-expression was RING-dependent (except for dkk1, see Discussion). nβ-gal mRNA was coinjected as a lineage tracer, stained in red. Amounts of mRNAs (ng/embryo): nβ-gal, 0.06; β-globin, 4.0; Ldb1, 4.0; XRnf12, 2.0 or 4.0; XRnf12 constructs coinjected with Ldb1, 1.0 or 2.0. Image published in: Hiratani I et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue gsc goosecoid, gsc-a, gsc-b, Xgsc Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone chrd chd, chordin, chrd.1, LOC108716939, X-chordin Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone otx2 otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone pcdh8 papc, paraxial protocadherin, xpapc Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone cer1 cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 Xenopus Sometime during NF stage 10 to NF stage 12.5 dorsal marginal zone foxp1 12cc4, fkh1b, hspc215, qrf1, xlfoxp1 Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone not flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone dkk1 dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 Xenopus Throughout NF stage 10 to NF stage 12.5 dorsal marginal zone tbxt bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury Xenopus Throughout NF stage 10 to NF stage 12.5 marginal zone mix1 mix.1, mix.2, mix2, tMix Xenopus Throughout NF stage 10 to NF stage 12.5 endomesoderm Image source: Published Larger Image Printer Friendly View

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