XB-IMG-23468
Xenbase Image ID: 23468
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Fig. 4. Effect of Mix.1 and Mix.2 loss of functions upon expression of nephrogenic mesoderm markers. (A) In situ hybridization analyses of XPax-8 or Xlim-1 expression in response to morpholino injection, or repression of Mix.1 target genes with enRMix.1. Morpholinos or enRMix.1 expression was targeted to the right side of the embryo. β-galactosidase was co-expressed and revealed with Red Gal. Mo1, Mo3 and enRMix.1 all cause a severe reduction of XPax-8 and Xlim-1 expression, showing that Mix.1 is required for the establishment of nephrogenic mesoderm. Neural expression of XPax8 in the otic placode (arrowhead) is also affected. In contrast, Mo2 has only a very limited effect on the expression of these markers, showing that Mix.2 alone is dispensable. (B) In situ hybridization analyses of mesodermal markers expression in response to Mo3 injection. Mo3 was targeted to the right side of the embryo (XmyoD, XPax-8, XOsr2) or ventrally (SCL). Although XPax-8 expression is inhibited, XmyoD expression is only slightly expanded on the injected side. XOsr2 expression is also inhibited in lateral mesoderm. In a minority of cases, SCL expression is reduced. Image published in: Colas A et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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