Xenbase Image ID: 23472

Fig. 8. Inhibition of FGF signalling rescues expression of nephrogenic mesoderm markers in Mo1 and Mo3-injected embryos. (A) Design of the experiment. Morpholinos were injected in the two right blastomeres at the 4-cell stage. At the mid-gastrula stage, a solution of the FGF receptor inhibitor SU5402 was injected into the blastocoel. Embryos were further cultured until the neurula stage, and processed for in situ hybridization analyses of XPax-8 and Xlim-1 expression. (B) Examples of rescue. Left column corresponds to morphant embryos that received intrablastocoelic injection of the solvent alone. These embryos display a strong inhibition of XPax-8 and Xlim-1 expression by Mo3. Right column shows rescue of XPax-8 and Xlim-1 by intrablastocoelic injection of the SU5402 solution. (C) Percentage of embryos with normal levels of XPax-8 or Xlim-1 expression on both sides relative to the total number of analyzed embryos (n) scored in three independent experiments. Image published in: Colas A et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 16 mesoderm dorsal otic placode intermediate mesoderm pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 17 mesoderm intermediate mesoderm Image source: Published Larger Image Printer Friendly View

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