Xenbase Image ID: 23473

Fig. 9. Perturbation of FGF signals during gastrulation by Mix.1 overexpression. High dose of Mix.1 mRNA (100 pg) was injected into the two right blastomeres at the 4-cell stage, and embryos cultured until the mid-gastrula stage for in situ hybridization analysis of FGF-encoding genes (FGF3, 4, 8) or FGF-target genes (Xbra and XmyoD). Expression of all analyzed genes is inhibited indicating that Mix.1 overexpression globally interfered with FGF signals in the marginal zone. At a lower dose that does not block gastrulation (25 pg), Xbra is still down-regulated. Embryos of the same series of injection fixed at later stages express XPax-8 at the neurula stage and XSMP30 at the tailbud stage, showing that pronephros development has not been affected. Image published in: Colas A et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 marginal zone involuted dorsal mesoderm involuted ventral mesoderm rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 33 and 34 pronephric kidney pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 13 mesoderm pronephric kidney otic placode intermediate mesoderm myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 11 marginal zone involuted dorsal mesoderm fgf3.S fgf-3, INT-2, int-2, xfgf3 X. laevis Throughout NF stage 11 mesoderm marginal zone involuted dorsal mesoderm involuted ventral mesoderm fgf4.L efgf, fgf-4, fgf4-a, fgf4-b, fgfe, HBGF-4, XEFGF, Xfgf-4, Xfgf4 X. laevis Throughout NF stage 11 marginal zone fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 11 marginal zone involuting marginal zone mesoderm Image source: Published Larger Image Printer Friendly View

Return to previous page