Xenbase Image ID: 23488

Figure 3. Pax3 Overexpression Increases Neural Crest Formation In Vivo, whereas Depletion of Pax3 Activity In Vivo Prevents Neural Crest Formation(A) Injections of 50–100 pg of Pax3 increase Slug (a), Snail (b), and FoxD3 (c) at the neural border as does ZicR1 (500 pg, d). In contrast, coinjections of Pax3 + ZicR1 expand NC at the neural border (not shown) and induce ectopic ventral Slug expression when targeted ventrally (e, ventral views). a–d, dorsal views.(B) Pax3-MO blocks Slug (a) and FoxD3 induction (b). In contrast, Pax3 mRNA is stabilized by the binding of the MO, resulting in an increased ISH signal (c), and Msx1 is unaffected (d).(C) Tadpole phenotype after Pax3 knockdown. Twist expression is strongly decreased (a and b, frontal views), embryos show fin and craniofacial defects (c), and pigment cells do not differentiate after bilateral injections (d amd e, albinos eggs fertilized with sperm from pigmented male).(D) Pax3-MO injection phenotype is dose dependent. More than 80% of the embryos show decreased NC markers expression at doses above 30 ng (red square). An MO with two nucleotide mismatch (Pax3-MO-mis, open squares) downregulates Slug expression in only 30% of the embryos, and the control-MO has no effect (circles). Mouse Pax3 mRNA rescues the knockdown phenotype (rescue, black circles): ISH for Slug (a) and FoxD3 (b). Image published in: Monsoro-Burq AH et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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