Xenbase Image ID: 23572
Figure 1. Functional Screening for Zygotic Nuclear Factors that Inhibit Activin-Induced Mesoderm Differentiation in the Animal Cap(A) Schematic of the functional screen.(B) q-PCR analysis for Xbra expression in Activin-treated animal caps injected with pooled mRNAs. In this experiment, pool #105 substantially suppressed Xbra expression.(C) Example of the isolation of a clone (#105-F5) that reduced Xbra expression. Each clone from the #105-F pool (12 clones) was analyzed as in (B).(D) The structure of XFDL. Nuclear localization signals (gray boxes); zinc-finger motifs (black boxes).(E) XFDL expression analyzed by q-PCR. Numbers represent the stages of embryos. E: unfertilized egg. (F–J) Whole-mount in situ hybridization of XFDL expression in 8-cell (F), late blastula (stage 9.5; G), early gastrula (stage 10.5; H and I), and mid-gastrula (stage 12; J) embryos. (I) Sagittal section. An: animal, Veg: vegetal, A: anterior, P: posterior, D: dorsal, V: ventral. (K) Immunohistochemistry using an anti-XFDL156 antibody (red) in animal cap cells at stage 10.5. DAPI staining (green).(L–Q) Expression of Xbra (L–N) and Mix.2 (O–Q) in animal cap explants analyzed by in situ hybridization. Animal caps given injections of control (L, M, O, and P; 400 pg/cell) or XFDL (N and Q; 400 pg/cell) mRNAs were prepared at the mid-blastula stage (stage 8.5) and cultured until siblings reached stage 11 without (L and O) or with (M, N, P, and Q) 5 ng/ml Activin.
Image published in: Sasai N et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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