Xenbase Image ID: 23601

Fig. 4. XFast-3 is expressed in a narrow window at early and mid-gastrulation. (A) Total RNA was extracted from embryos at the times indicated and analysed by RNase protection using probes against XFast-1, XFast-3 or FGFR (loading control). The protected fragments are indicated, as are the undigested probes. The lane marked tRNA is a control for probe digestion. (B) XFast-1 and XFast-3 are expressed strongly in the animal cap and in the prospective mesoderm of stage 10.25 embryos. The embryos were bisected through the dorsal lip before in situ hybridisation using probes against XFast-1 or XFast-3. Specific staining is blue, and is strongest in the prospective mesoderm and ectoderm. The dark brown colour is the pigmented animal cap. Image published in: Howell M et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue foxh1.2.L fast3, XFast-3 X. laevis Throughout NF stage 10.25 animal cap involuted ventral mesoderm involuted dorsal mesoderm foxh1.L fast-1, fast1, foxh1a, LOC108719776, XFast-1, xfast1, xfoxh1 X. laevis Throughout NF stage 10.25 animal cap involuted dorsal mesoderm involuted ventral mesoderm Image source: Published Larger Image Printer Friendly View

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