Xenbase Image ID: 23652

Fig. 8. FoxC2 induces ectopic expression of paraxial genes in the intermediate mesoderm. Chick embryos were electroporated with cFoxC2 (A–D and F–I) and analyzed for expression of Paraxis (C–D) and Pax-7 (H and I). The same embryo is shown by brightfield (A), fluorescence to detect GFP (B), and transcript for Paraxis (C), while D is a separate embryo showing Paraxis expression only. Control embryo in E without electroporation, hybridized for Paraxis in the same vial as embryos in C and D. Note the expansion of Paraxis expression into the IM adjacent to the somites (arrows, C and D) when compared to control (E). (F–H) View of the same microscopic field illustrates nuclei (DAPI, F), GFP (G), and Pax7 (H). (I) Different electroporated embryo depicting Pax7 only. Note the expansion of Pax7 into the IM (*) but not into the lateral plate (H and I) when compared to controls (J and K). Abbreviations as in Fig. 7; lp, lateral plate. Image published in: Wilm B et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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