Xenbase Image ID: 23736

Fig. 8. Xmxi1 induces proliferation in the open neural plate of Xenopus embryos. MT-Xmxi1 (500 pg), EnR-Xmxi1 (500 pg), and Xmxi1-GR (500 pg, induced at stage 10.5 with dexamethasone), Xmxi1-MO (12.5 ng), or the control-MO (Co-Mo) (12.5 ng) was injected in Xenopus embryos. Proliferation on the injected side compared with the noninjected side was measured by counting (A) pH3 or (B) BrdU-positive cells of 15 consecutive sections of five embryos in the open neural plate region. Shown is the average number of positive cells per section. Error bars indicate the standard error of the mean. (C) Forced cell cycle arrest does not alter the phenotype of Xmxi1-injected embryos. In Xmxi1-GR-injected embryos, proliferation was blocked by HUA treatment at stage 10 and induced at stage 10.5 with dexamethasone. (D) Inhibition of MT-XMxi1-induced apoptosis does not rescue loss of N-tubulin. Overexpression of MT-Xmxi1 (500 pg) led to an increase in apoptosis as seen by TUNEL staining (upper panel, right). Coinjection of human Bcl2 mRNA (500 pg) blocked apoptosis (lower panel, right) but did not influence the effects of MT-Xmxi1 on Sox3 and N-tubulin. Image published in: Klisch TJ et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 13 neural plate trigeminal placode tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 13 chordal neural plate neuron lateral Image source: Published Larger Image Printer Friendly View

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