Xenbase Image ID: 23740

Fig. 3. SRp38 can inhibit mesodermal differentiation. Control embryos on left. SRp38 injection (250 pg each) on right. Some embryos are also stained for lacZ activity (in red) as a lineage tracer. All injection sites are marked with black arrowhead. (A-C) Early mesoderm injections were targeted toward ventral domains. In situ hybridization for Bix1, stage 9.5 (A); brachyury, stage 11 (B); and wnt8, stage 11 (C). SRp38 injection (250 pg each, right) does not inhibit expression of Bix1 but does inhibit expression of Brachyury and wnt8. (D) In situ hybridization for myoD, stage 25. Dorsal injection of SRp38 (250 pg each, right) inhibits expression of myoD at the site of injection (marked by red tracer). (E) In situ hybridization for α-globin, stage 25. Ventral injection of SRp38 (250 pg each, right) inhibits expression of α-globin at the site of injection. (F) Tor70 antibody reveals disrupted notochord development in embryos injected dorsally with SRp38 (two sections on right, notochord indicated by red arrowhead). Image published in: Liu KJ and Harland RM (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10 mesoderm marginal zone bix1.3.L bix1, bix4 X. laevis Throughout NF stage 9 mesoderm marginal zone wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Throughout NF stage 11 mesoderm dorso-lateral marginal zone myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 25 mesoderm paraxial mesoderm somite presomitic mesoderm hba1.S alpha globin, alpha-globin, T3 globin X. laevis Throughout NF stage 25 ventral blood island ventral hematopoietic stem cell Image source: Published Larger Image Printer Friendly View

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