Xenbase Image ID: 23743

Fig. 6. SRp38 is required for regulated neurogenesis. (A) Dorsal views, in situ hybridization for neuronal specific β-tubulin. Top, left to right: control embryos show expression of β-tubulin in neurons and trigeminal ganglia; SRp38-injected embryos show a loss ofβ -tubulin-expressing cells; injection of antisense morpholino oligonucleotides (AMOs) does not perturb expression ofβ -tubulin. Bottom, left to right: inhibitory Delta (Deltastu) injection results in increased and disorganized expression of β-tubulin; co-expression of Deltastu and SRp38 results in almost normal embryos (compare with +SRp38 above); co-injection of Deltastu and AMOs results in increased expression of β-tubulin (compare with +AMO embryo above and to controls). (B) In situ hybridization for nrp1, dorsoanterior view, stage 17. Left: control embryos show nrp1 staining in the neural plate and eye primordia. Middle: SRp38 overexpression inhibits expression of nrp1, red arrows. Right: co-injection of SRp38 with the function-blocking sequence C3 rescues expression of nrp1, red arrows. (C) RT-PCR analysis on animal cap ectodermal explants. Expression of 100 pg of neurogenin in the animal cap results in expression of neuronal β-tubulin and a mild decrease in Id3 (lane 3). Co-expression of neurogenin and 5ng of C3 results in a greater increase of neuronalβ -tubulin and concomitant decrease in Id3 (compare control lane 5 with lane 3). C3 alone (lane 6, 5 ng) results in complete loss of Delta and no effect on Id3 expression. CyclinD1 and p27xic1 expression are unchanged in neuralized explants upon addition of C3 (compare lane 5 to lane 4). (D) TUNEL staining indicates apoptotic cells. Dorsal views of stage 15 embryos. Control embryos show very few TUNEL-positive cells (black arrowhead), while embryos injected with 250 pg of SRp38 RNA in one cell at the two-cell stage show a variable, though clear increase, in the number of TUNEL-positive cells (red arrowheads). (E) Primary neurogenesis is increased in the absence of SRp38 and Delta function. Image published in: Liu KJ and Harland RM (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nrp1 A5 antigen, LOC108718763, neuropilin, neuropilin-1, np-1, npn-1, nrp, nrp-1, pNPG152 Xenopus Throughout NF stage 17 neural plate eye primordium pre-chordal neural plate anterior neural fold Image source: Published Larger Image Printer Friendly View

Return to previous page