XB-IMG-23881
Xenbase Image ID: 23881
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Fig. 7. The effects of maternal XTcf4 depletion. (A) Wnt target gene expression in whole embryos at the late blastula (stage 9.5) and early gastrula stage (stage 10.5). Real time RT-PCR showing the relative expression of chordin, Xhex, siamois, and Xnr3. Embryos were uninjected controls (uninj), injected with 4 ng XTcf4.1 oligo (XTcf4–), or injected with 4 ng XTcf4.1 oligo and 48 h later with 12.5 pg XTcf4A mRNA (XTcf4–+RNA). XTcf4 depletion causes a loss of expression of Wnt target genes, particularly chordin, Xhex, and Xnr3, changes that are partially rescued by the re-introduction of XTcf4 mRNA. (B) The phenotypes of tailbud stage embryos injected as oocytes with 7 ng XTcf4.1 oligo (red) compared to uninjected control siblings (brown). The ventralized phenotype is partially rescued by the introduction of 12.5 pg XTcf4A + 4B mRNA (blue). (C) Chordin expression extends further ventrally in XTcf1-depleted embryos (center) than in uninjected controls (left). Chordin expression is greatly diminished in XTcf4-depleted embryos (right). Vegetal view of stage 10.5 embryos, dorsal at top. (D) Chordin expression is much diminished in XTcf1-depleted early gastrulae compared to uninjected controls at the early gastrula stage. (E) Vegetal view of stage 10.5 control embryos (left) and XTcf1− embryos (right), dorsal at top. β-galactosidase mRNA injected into cell B1 at the 32-cell stage marks dorsal tissue in the gastrula, visualized in red. The red stain colocalizes with the most intense expression of chordin, visualized in blue, in all the embryos, showing that there has been no reversal of dorsoventral polarity as a result of XTcf1 depletion. Image published in: Standley HJ et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
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