Xenbase Image ID: 23917

Fig. 2. Hairy2 is required for NC induction downstream of FGF and BMP. Embryos were injected either with mRNA encoding an activated form of FGFR4 (torso fused FGFR4, tFGFR4) and a control MO or with tFGFR4 mRNA and Hairy2 MOs. tFGFR4 and CoMO injections expanded Snail2 (a) and FoxD3 (c) (Expansion in 62% and 43%, n = 24 and n = 2 8 respectively). Hairy2 MOs blocked the ability of tFGFR4 to expand Snail2 (b) and FoxD3 (d) (61%, n = 28 and 63% decreased, n = 27 respectively). Attenuation of BMP signaling by tBR with CoMO expanded Snail2 (e) and FoxD3 (g) (expansion in 68%, n = 19 and 60%, n = 20 respectively). In the presence of Hairy2 MOs, tBR no longer induced expansion of Snail2 (f, decreased in 35%, n = 20) nor FoxD3 (h, decreased in 73%, n = 19). Wnt signaling with CoMO expanded both Snail2 (i, 84%, n = 19) and FoxD3 (k, 83%, n = 18). Co-injection of Wnt7b and Hairy2 MOs still expanded both Snail2 (j, 78% expansion, n = 18) and FoxD3 (l, 100% expansion, n = 19). All embryos are shown in dorso-anterior view. β-galactosidase was used as a lineage tracer (red staining) and the injected side is oriented to the right. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 17 neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 17 neural crest Image source: Published Larger Image Printer Friendly View

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