Xenbase Image ID: 23920

Fig. 5. Hairy2 negatively regulates Id3 by repression of BMP4. (A) At neurula stage, Hairy2 and Id3 expression overlaps in the NC region as shown by double in situ hybridisation. Id3 is in purple and Hairy2 in dark blue (a–b). Higher magnifications is shown (a’–b’). (B) Hairy2-GR overexpression (500 pg) decreases Id3 (a, 52%, n = 25). Knockdown of Hairy2 increases Id3 (b, 56%, n = 32). Overexpression of a Hairy2 DNA-binding mutant (Hairy2DBM-GR 500 pg) does not affect Id3 (c, 95% unaffected, n = 22). In NC induced animal caps, Hairy2 MOs (5 ng/blastomere) upregulates Id3 while Hairy2-GR overexpression (250 pg/blastomere) reduces Id3. Hairy2DBM-GR (250 pg/blastomere) has no effect on Id3 (d, e). Id3 overexpression or depletion does not affect Hairy2 expression significantly (f, g, 90% n = 19 and 80% n = 24, respectively). (C) Hairy2-GR overexpression inhibits (a, 56%, n = 18), while its knockdown increases BMP4 expression in the NC (b, 45%, n = 22). Overexpression of Hairy2DBM-GR (500 pg) has no effect on BMP4 expression in NC (c, 80% normal, n = 15). Q-PCR analysis of animal caps induced to NC injected with Hairy2 MOs, Hairy2-GR or Hairy2DBM-GR (d, e). Injection of CA-Alk3 (25 pg) activates Id3 (f, 43%, n = 16) and blocks Hairy2 ability to repress Id3 in embryos (g, 77% normal, n = 25). All embryos are shown in dorso-anterior view. β-galactosidase was used as a lineage tracer (light blue staining) and the injected side is oriented to the right. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue hes4.S H2, hairy2, hairy2a, hairy2b, hes4-a, hes4-b, hes4a, hes4b, Xhairy2, XHairy2a, Xhairy2b X. laevis Throughout NF stage 13 to NF stage 15 neural crest anterior neural fold neuroectoderm id3.L heir-1, id3-a, id3-b, xid3, xidia, xIdx X. laevis Sometime during NF stage 13 to NF stage 15 neural crest anterior neural fold neural plate neural groove neuroectoderm bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 15 neural crest cement gland primordium cardiac mesoderm Image source: Published Larger Image Printer Friendly View

Return to previous page