Xenbase Image ID: 23921

Fig. 6. Id3 interacts and blocks Hairy2 activity in NC. (A) Overexpressed Flag-tagged Hairy2 but not Hairy1, Hes2 or Hes6 interacts with HA-tagged Id3 in immunoprecipitation assays. (B–C) Mapping of the domains of Hairy2 and Id3 required for their interaction. Note that only the Hairy2 deletion mutants containing the Orange domain are able to interact with Id3 (B) and that the C-terminal part of the HLH domain of Id3 is required for Hairy2 interaction (C). (D) Co-injection of Id3-GR (1 ng) rescues Snail2 inhibition by Hairy2-GR (70% rescued, n = 26)(a, b). Q-PCR analysis of Snail2 in animal caps induced to form NC injected radially with Hairy2-GR (250 pg), Id3-GR (500 pg) or both (250 pg and 500 pg respectively. A control western blot showing that in each condition similar levels of overexpressed proteins Myc-Hairy2-GR and Flag-Id3-GR have been produced is shown (c). Co-injection of Id3-GR (1 ng) blocks the ability of Hairy2-GR to induce SoxD (d, 89% ectopic n = 18; e, 74% rescued n = 19). Dorso-anterior views with injected side to the right (a, b) or lateral views (d–g) of neurula embryos are shown. β-galactosidase was used as a lineage tracer. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 19 neural crest Image source: Published Larger Image Printer Friendly View

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