Xenbase Image ID: 23927

Fig. 4. Hairy2 promotes NC markers and proliferation through Delta1 activation. (A) Q-PCR analysis of Sox10, Snail2 and SoxD expression in NC induced intact or dissociated animal caps derived from Hairy2-GR mRNA (250 pg/blastomere) injected embryos (−, Hairy2-GR mRNA injected minus DEX). Sox10 induction observed upon Hairy2-GR overexpression in intact stage 28 NC induced caps is not observed in dissociated NC induced explants (a). However, repression of Snail2 or induction of SoxD still occurs in dissociated stage 14 NC induced caps (b,c). (B) Whole-mount in situ hybridization of embryos co-injected with Hairy2-GR and Delta1Stu mRNA (1 ng) or Delta1 MO (7.5 ng). Co-injection of Hairy2-GR with Delta1Stu mRNA (1 ng) or Delta1 MO (7.5 ng) affects its ability to induce Sox10 in tailbud embryos, but not its capacity to repress Snail2 or activate SoxD in neurula embryos. Respective repression: (a) 63%, n = 23; (b) 43%, n = 21; (c) 64%, n = 24 and (d) 65%, n = 20. Respective induction: (e) 87%, n = 16 and (f) 60%, n = 28. Lateral views with control and injected sides (a, b, e, f) or dorsal views (c, d) with the injected side on the right are shown. LacZ was used as lineage tracer. (C) Percentage of embryos injected with Hairy2-GR mRNA, Hairy2-GR plus Delta1Stu mRNA (1 ng) or Delta1 MO (7.5 ng) showing increased pH3 staining. Note that co-injection of Delta1Stu mRNA or Delta1 MO blocks Hairy2 ability to promote cell proliferation (−, Hairy2-GR mRNA injected minus DEX). Number of embryos examined is indicated. (D) Percentage of embryos injected as indicated with Bax mRNA (50 pg), Hairy2-GR mRNA (500 pg), Delta1Stu (1 ng) or Delta1 MO (7.5 ng) developing normally or showing a low or high number of dying cells at gastrula stage as shown in Fig. 1C (−, Hairy2-GR mRNA injected minus DEX). Note that Hairy2-GR rescues Bax mRNA mediated embryonic lethality when Notch signaling is blocked. Number of embryos examined is indicated. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox10 Xenopus Throughout NF stage 28 neural crest otic vesicle forebrain branchial arch branchial arch 1 branchial arch 4 snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 Xenopus Throughout NF stage 14 neural crest sox15 sox-d, sox20, sox26, sox27, soxd, xsoxd Xenopus Throughout NF stage 14 neural crest neuroectoderm pre-chordal neural plate Image source: Published Larger Image Printer Friendly View

Return to previous page