XB-IMG-23928
Xenbase Image ID: 23928
Fig. 5. Rescue of Hairy2 depletion by Delta1. (A) Whole-mount in situ hybridization of embryos injected with Hairy2 MOs (10 ng) alone or with Delta1 mRNA (1 ng) as indicated. Delta1 restores Snail2 (a, b) and Sox10 (c, d) expression in Hairy2 MOs injected embryos. Respective inhibition: (a) 76%, n = 17; (b) 30%, n = 24; (c) 50%, n = 16; (d) 15%, n = 28. Dorsal views of neurula embryos (a, b) with the injected side on the right or lateral views of tailbud embryos (c, d) with control and injected sides revealed by LacZ staining are shown. (B) Percentage of embryos injected with Hairy2 MOs (10 ng) alone or together with Delta1 mRNA (500 pg) showing reduced pH3 staining. Delta1 injection partially rescues the inhibition of proliferation observed in Hairy2 depleted embryos (−, rhodamine dextran injected alone). Number of embryos examined is indicated. (C) Whole-mount in situ hybridization of tailbud embryos injected with Hairy2 MOs (10 ng) with Su(H)Ank-GR mRNA (500 pg). Su(H)Ank-GR efficiently restores Sox10 expression in Hairy2 MOs injected embryos. Rescue of Sox10 is not observed in − DEX control embryos. Respective inhibition: a, 77% n = 18; b, 42% n = 21. Lateral views with control and injected sides identified by LacZ staining are shown. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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