XB-IMG-23930
Xenbase Image ID: 23930
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Fig. 7. Stat3 is necessary for Hairy2 non-cell-autonomous function. (A) Whole-mount in situ hybridization of embryos injected with Hairy2-GR mRNA (500 pg), Stat3 MOmis (15 ng) or Stat3 MO (15 ng) as indicated. Note that injection of Stat3 MO blocks Hairy2 ability to induce Delta1 (a, 70% induced, n = 22; b, 20%, induced, n = 20) and to upregulate Sox10 (c, 55% increased, n = 30; d, 12% increased, n = 26) but not its ability to induce SoxD (e, 90% increased, n = 20; f, 82% increased, n = 22). Dorso-anterior views of neurula embryos (a,b) with the injected side on the right or lateral views of embryos (c–f) with control and injected sides are shown. LacZ mRNA was co-injected as a lineage tracer to identify the injected side. (B) Percentage of embryos injected with Hairy2-GR mRNA (500 pg), Stat3 MO7mis (15 ng) or Stat3 MO (15 ng) as indicated showing increased pH3 staining. Note that the co-injection of the Stat3 MO reduces Hairy2 ability to stimulate cell proliferation (−, Hairy2-GR mRNA injected minus DEX). Number of embryos examined is indicated. (C) Whole-mount in situ hybridization of embryos injected with Hairy2-GR (150 pg) or Stat3-GR (500 pg) mRNA as indicated. Note that Hairy2-GR or Stat3-GR mRNA injection has no effect on Delta1 expression (a, none affected n = 12; b, 0%, n = 15). When combined, Delta1 is however strongly upregulated (c, 71% induced, n = 21). Hairy2-GR (50 pg/blastomere) with Stat3-GR (250 pg/blastomere) also strongly increases Delta1 in NC induced animal caps. The amount of myc-tagged Hairy2-GR and Stat3-GR proteins detected using anti-myc and anti-Stat3 proteins produced in each condition is shown (d). Dorsal views of neurula embryos with the injected side on the right are shown. LacZ was co-injected to reveal the injected side. Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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