Xenbase Image ID: 2394

Fig. 2. Macroarray screen of the maternal β-Catenin pathway. (A) xy-scatter plot of the intensities of the individual spots after hybridization with “LiCl” and “δN-XTcf-3” probes. Clones are categorized into five regions (I–V) depending on the extent of regulation by the maternal β-Catenin pathway. Note that chordin and Xnr-3 comprise most of the clones strongly regulated by β-Catenin (region V). (B–G) Whole-mount in situ hybridization of gastrula stage embryos left untreated (B, D, F) or treated with LiCl (C, E, G) and hybridized with xHAS-rs (B, C), XFD2/FoxI1a (D, E), and S10-45-H5 (F, G) probes. All embryos are shown from the side with dorsal to the right. (H) RT-PCR analysis of whole gastrula stage embryos injected radially with δN-XTcf-3 (800 pg, lane 2), siamois-ENR (120 pg, lane 3), and a combination of δN-XTcf-3 and siamois (200 pg, lane 4). EF1-α serves as RNA loading control. Image published in: Wessely O et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ptmap12.L LOC728026, LOC734001 X. laevis Throughout NF stage 10 animal cap foxi1 ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 Xenopus Throughout NF stage 10 ectoderm animal cap has-rs.S hasrs X. laevis Throughout NF stage 10.5 dorsal marginal zone ectoderm Image source: Published Larger Image Printer Friendly View

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