Xenbase Image ID: 23943

Fig. 8. FISH and immunohistochemistry for NF-M. (A,A′) NF-M FISH of single confocal optical sections from opposite sides of spinal cord or unilaterally injected hnRNP K MO1 tadpole (stage 39/40). Broken outlines surround individual neurons. (B1-D4) Cells from dissociated cell culture. (B1-B3) Control MO neuron stained for NF-M protein (B1), RNA (B2) and RNA/DAPI merged (B3). (C1,C2) Adjacent cells from a unilaterally injected hnRNP K MO culture, viewed for NF-M protein (C1) and RNA (C2). Arrow indicates normal staining for both protein and RNA; arrowhead indicates a cell with no protein and FISH pattern typical of hnRNP K MO-injected spinal cord neurons from whole mount. (D1-D4) Neurons from a bilaterally injected hnRNP K MO culture stained for protein (D1), RNA (D2), DAPI (D3) and RNA/DAPI merged (D4). Scale bars in B1,C1 and D2 apply to B1-3,C1-2 and D1-4, respectively. Bar graph shows qRT-PCR of nuclear and cytoplasmic fractions for NF-M and peripherin RNAs from bilaterally injected hnRNP K MO and control animals assayed at stage 29/30. δCT, mean (±s.d.) difference in the number of PCR cycles to reach threshold (see text). *δCT was significantly less for NF-M RNA in hnRNP K MO embryos than for other groups (P<0.01, one-way ANOVA). Image published in: Liu Y et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nefm.L nef-m, nef3, nefm-a, nefm-b, nf-m X. laevis Throughout NF stage 39 to NF stage 40 spinal cord Image source: Published Larger Image Printer Friendly View

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