Xenbase Image ID: 23950

Figure 5. Excessive Doses of ONT1 Cause Dorsalization in the Embryo(A and B) The dorsalized phenotype was observed following the injection of ONT1 mRNA (200 pg; [B]).(C–F) Microinjection of ONT1 mRNA (200 pg) increased the dorsal markers Gsc ([C and D]; anterior view) and Chordin (not shown) and suppressed the ventral marker Szl ([E and F]; vegetal view).(G) Embryos were classified by morphological inspection of (H)–(L).(H–L) Stage 23 embryos received injections of BMP1 mRNA (200 pg) or ONT1 mRNA (400 pg) into the two dorsal blastomeres at the four-cell stage. Overexpression of ONT1 reversed the ventralization induced by BMP1 (I and J) but not a constitutively active form of BMPR (CA/BMPR; 6.25 pg; [K and L]).(M–O) The dorsalizing effect of ONT1 overexpression requires Chordin. In situ hybridization for the indicated markers in a control embryo (M) or in embryos given an injection of Chordin-MO (4 ng each; N) or ONT1 mRNA (200 pg) + Chordin-MO (4 ng each) (O). Image published in: Inomata H et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue szl.L frzb3, secreted frizzled, sizzled, xszl X. laevis Throughout NF stage 13 ventral marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 13 dorsal marginal zone otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 23 optic vesicle forebrain myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 23 somite trunk somite paraxial mesoderm presomitic mesoderm egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 23 brain hindbrain cranial neural crest rhombomere R3 rhombomere R5 szl.L frzb3, secreted frizzled, sizzled, xszl X. laevis Throughout NF stage 23 ventral blood island Image source: Published Larger Image Printer Friendly View

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