XB-IMG-23953
Xenbase Image ID: 23953
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Fig. 1. Expression patterns of Xenopus laevis Irx genes during pronephros development. Embryos are shown in lateral views (except when indicated); red arrowheads indicate the kidney territory. (A-D) Expression pattern of Irx1 at indicated developmental stages. At mid-(A) or late (B) neurula, Irx1 is detected in the dorsal pronephric territory. Insets show pronephric territory of an embryo double-stained for Lim1 or Pax8 (blue) and Irx1 (purple). Irx1 expression is restricted dorsally. During tailbud (C) or tadpole (D) stages, Irx1 expression shifts to a more ventral region that will form the intermediate tubule. Inset in C indicates an embryo double-stained for Pax8 (blue) and Irx1 (purple). Note the ventral position of Irx1 in the future intermediate tubule. Inset in D indicates a higher magnification of the pronephric Irx1 territory. Note the expression of Irx1 in the migrating ventral mesoderm (blue arrowheads). (E-H) Irx2 shows an expression pattern similar to that of Irx1, although it is not expressed in ventral migrating mesoderm. (I-L) Spatial distribution of Irx3 mRNA. (I) At mid-neurula, Irx3 mRNA is detected in a broad domain that contains most of the pronephric territory. Inset indicates pronephric territory of an embryo double-stained for Lim1 (blue) and Irx3 (purple). (J) At late neurula/early tailbud stages, Irx3 becomes restricted to the ventral pronephric territory. Inset indicates Irx3 ventral restriction in an embryo co-stained for Pax8 (blue). (K,L) From tailbud stages, Irx3 expression is detected in the intermediate tubule. Inset in K indicates embryo double-stained for Pax8 (blue) and Irx3 (purple). Inset in L indicates high magnification of the pronephric Irx3 territory. (M,N) Lateral view (M) and transverse section (N) of late neurula embryos showing Lim1 (blue) and Irx1 (purple) expression. Irx1 expression is restricted to the dorsal pronephric anlage. (O,P) Double staining for Sglt1k (green) and Irx1 (red) in tailbud (O) or tadpole (P) embryos. Insets indicate single Irx1 red channel. The Irx1 expression domain is located just distal to that of Sglt1k. (Q,R) Lateral view (Q) and transverse section (R) of late neurula embryos showing Lim1 (blue) and Irx3 (purple) expression. There is initial broad expression of Irx3 in most of the pronephric anlage (Q) and a later restriction to the ventral pronephros (R). (S,T) Double staining for Nkcc2 (green) and Irx3 (red) in tailbud (S) or tadpole (T) embryos. Insets show single Irx3 red channel. The expression domains of both genes largely overlap, but the Irx3 domain extends proximally into the proximal tubule, whereas Nkcc2 extends distally into the distal tubule. Image published in: Alarcón P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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