Xenbase Image ID: 23957

Fig. 5. Early Irx gene requirement for pronephros development occurs at neurula stages. Embryos are shown in lateral views. Embryos were injected in a single blastomere (V2.2) at the 8- to 16-cell stage and lacZ mRNA was used as linear tracer. Late neurula-early tailbud Xenopus embryos co-injected with 500 pg of HD-E1A-GR (A-H), HD-GR (I-P) or HD-EnR-GR (I,M, inset) mRNAs and assayed for expression of Lim1. (A-H) Embryos injected with a hormone-inducible activating form of Irx (HD-E1A-GR) show expanded Lim1 only when Dex was added during mid neurula stages (arrowheads). (I-P) Embryos injected with a hormone-inducible dominant negative (HD-GR) form of Irx show reduced Lim1 expression only when Dex was added during mid neurula stages (arrowheads). The same results were found with a hormone-inducible repressing form of Irx (HD-EnR-GR) (I and M, inset and not shown). Image published in: Alarcón P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue lhx1 lim-1, Lim1, lim1, Xlim-1, Xlim1 Xenopus Throughout NF stage 15 pronephric mesenchyme neural tube lhx1 lim-1, Lim1, lim1, Xlim-1, Xlim1 Xenopus Throughout NF stage 17 pronephric mesenchyme neural tube lhx1 lim-1, Lim1, lim1, Xlim-1, Xlim1 Xenopus Throughout NF stage 20 pronephric mesenchyme early proximal tubule lhx1 lim-1, Lim1, lim1, Xlim-1, Xlim1 Xenopus Throughout NF stage 22 pronephric mesenchyme early proximal tubule neural tube Image source: Published Larger Image Printer Friendly View

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