Xenbase Image ID: 23958

Fig. 6. Irx gene loss of function kidney defects are partially rescued by increased Smad1 activity in Xenopus. Embryos are shown in lateral views and red arrowheads indicate the kidney territory. Embryos were injected in a single blastomere (V2.2) at the 8- to 16-cell stage and lacZ mRNA was used as linear tracer. (A,B) Injection of 500 pg of Smad1GR mRNA, upon addition of dexamethasone (Dex) at stage 14, expanded Pax8 expression. No effect was observed in the absence of hormone (not shown). (C-F) In embryos co-injected with 500 pg of Smad1GR mRNA and 4 ng of each Irx1 and Irx3 MOs Pax8 expression was downregulated (C,D) or rescued (E,F) in the absence or presence of Dex, respectively. (G,H) Depletion of Irx1 and Irx3 impaired Bmp7 expression. Image published in: Alarcón P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax8 pax-8, XPax-8, XPax8 Xenopus Throughout NF stage 24 pronephric mesenchyme otic placode bmp7.1.L bmp-7, bmp7, op-1 X. laevis Throughout NF stage 32 retina branchial arch heart primordium pronephric nephrostome Image source: Published Larger Image Printer Friendly View

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