Xenbase Image ID: 23959

Fig. 7. Injection of different doses of Irx1 or Irx3 MOs reveal an early and a late requirement of this gene during pronephric development in Xenopus. Embryos are shown in lateral views and red arrowheads indicate the kidney territory. Embryos were injected in a single blastomere (V2.2) at the 8- to 16-cell stage and lacZ mRNA was used as linear tracer. (A-D) Injection of low doses (4 ng) of Irx1 MO had little effect on Sglt1k expression (A,B) but downregulated the proximal domain of Nkcc2 (C,D). (E-L) Injection of low doses (4 ng) of two different Irx3 MOs downregulated the distal expression of Sglt1k (E,F,I,J) and the proximal domain of Nkcc2 (G,H,K,L). No effect was observed in the duct, as determined by Gata3 expression (E,F,I,J). (M-P) Injection of high doses (8 ng) of MOIrx3.2 strongly downregulated Sglt1k (M,N) and Nkcc2 (O,P). Most injected embryos were malformed, as shown in O,P. Nevertheless, a few displayed normal morphology, like that shown in M,N. Image published in: Alarcón P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue gata3.S GATA-3, XGATA-3, XGATA3 X. laevis Throughout NF stage 35 and 36 pronephric duct otic vesicle midbrain-hindbrain boundary gill primordium slc12a1.L Na-K-2Cl cotransporter, NKCC-2, NKCC2, XNKCC2 X. laevis Throughout NF stage 35 and 36 pronephric kidney early distal tubule slc5a1.2.S D22S675, sglt-1, sglt-1k, sglt1 X. laevis Throughout NF stage 35 and 36 early proximal tubule late proximal tubule Image source: Published Larger Image Printer Friendly View

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