XB-IMG-2397
Xenbase Image ID: 2397
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Fig. 5. Macroarray analysis of dissected dorsal and ventral tissue. (A) Dorsal marginal zones (DMZ) and ventral marginal zones (VMZ) of stage 10.5 embryos were excised and dorsal- and ventral-specific probes prepared. (B) Graph of screen using DMZ and VMZ probes. Chordin and goosecoid were the two genes with greatest differential dorsal expression. The ventral genes XVent2, xHAS-1, and xHAS-rs are also indicated. The graph shows the analysis of a different filter from the one shown in the previous figures. (C–F) Whole-mount in situ hybridization of gastrula stage embryos with FRL-1 (C, D), B3C21 (E), and xPRMT1b (F) in lateral view (C, E, F) or vegetal view (D). (G) The 600 dorsal-most enriched spots were projected onto the combined organizer graph of Fig. 4. The results of three different differential screens of 18,000 gastrula cDNAs plated in duplicate onto a single nylon filter are integrated in this one graph. The wide range of expression of the 600 spots suggests that there are additional pathways besides β-Catenin and Nodal-related that affect dorsal-specific gene expression. Image published in: Wessely O et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V.
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