Xenbase Image ID: 24002

Fig. 1. Requirement of FoxM1 for both cell proliferation and expression of G2-M cell-cycle regulators in the Xenopus neural plate. (A) Embryos were analyzed for FoxM1 expression by either RT-PCR (upper panel) or WISH (lower panels). In RT-PCR analysis, EF1-α was used as a loading control; ODC was also used as a loading control and was confirmed to be expressed throughout embryogenesis (data not shown). UFE, unfertilized egg; N/F, Nieuwkoop-Faber. (B) Embryos injected with both lacZ mRNA (100 pg) and control MO or FoxM1-MO (18 ng) at one blastomere at the two-cell stage were cultured, fixed at st. 13, and analyzed by immunostaining with anti-pH3 antibody. A dorsal view of the embryos is shown (left panels, anterior up), with the injected side (β-Gal, light blue) being on the right of the midline (dotted). The area boxed in black is enlarged in the lower panels. (Right panel) Relative numbers of pH3-positive cells on the injected and uninjected sides of the neural plate (the area boxed in red) are shown, with the number on the uninjected side set at 1.0. Error bars indicate s.d. (n=10); *P<0.01. (C) Embryos co-injected with lacZ mRNA and either control MO or FoxM1-MO, as in B, were cultured until st. 14. Neural plates were isolated from these embryos, stained with Hoechst, and photographed (left panels) for Hoechst-stained nuclei (the injected side of the neural plate being on the right). (Right panel) Relative numbers of cells on the injected and uninjected sides of the neural plate are shown, with the number on the uninjected side set at 1.0. Error bars indicate s.d. (n=10); *P<0.01. (D) Embryos co-injected with lacZ mRNA and either control MO or FoxM1-MO, as in B, were fixed at st. 15-16 and analyzed by WISH for Cdc25B and cyclin B3. The injected side (β-Gal, red) is on the right. (E) Embryos pre-injected with control MO or FoxM1-MO (36 ng) at the one-cell stage were analyzed at st. 13 by either RT-PCR (upper panel) or immunoblotting (lower panel) for the indicated transcripts or proteins (EF1-α and ERK1 being loading controls). For a rescue experiment, embryos were co-injected with FoxM1-MO (36 ng) and FoxM1-MO-resistant FoxM1 mRNA (200 pg). Image published in: Ueno H et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue foxm1.S fkhl16, foxm1l, hfh-11, ins-1, LOC108710185, mpp-2, mpp2, Trident, WIN, xlFoxM1 X. laevis Throughout NF stage 10 animal hemisphere foxm1.S fkhl16, foxm1l, hfh-11, ins-1, LOC108710185, mpp-2, mpp2, Trident, WIN, xlFoxM1 X. laevis Throughout NF stage 14 neural plate foxm1.S fkhl16, foxm1l, hfh-11, ins-1, LOC108710185, mpp-2, mpp2, Trident, WIN, xlFoxM1 X. laevis Throughout NF stage 19 neural tube foxm1.S fkhl16, foxm1l, hfh-11, ins-1, LOC108710185, mpp-2, mpp2, Trident, WIN, xlFoxM1 X. laevis Throughout NF stage 25 head cdc25b.L cdc25b-a, cdc25b-b X. laevis Sometime during NF stage 15 to NF stage 16 neural plate ccnb3.S X. laevis Sometime during NF stage 15 to NF stage 16 neural plate Image source: Published Larger Image Printer Friendly View

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