Xenbase Image ID: 24004

Fig. 3. Requirement of FoxM1 for neuronal differentiation but not specification. (A) Xenopus embryos pre-injected with control MO or FoxM1-MO (36 ng) together with or without FoxM1 mRNA (200 pg) at the one-cell stage were cultured until st. 33 and photographed (left panels). For RT-PCR analysis, embryos were collected at st. 28 (right panel). (B) Embryos injected with lacZ mRNA (100 pg) and either control MO or FoxM1-MO (18 ng) at one blastomere at the two-cell stage were cultured until st. 14 for WISH analysis of Xngnr1, N-tubulin and MyoD, or until st. 16 for analysis of Sox2 and N-CAM. In each panel, the injected side (β-Gal, red) of the embryo is on the right (dorsal view, anterior up). (C) Animal caps from the late blastula embryos pre-injected with control MO or FoxM1-MO (36 ng) at the one-cell stage were cultured with or without Activin (200 pM) until sibling control embryos reached st. 20 and then analyzed by RT-PCR. (D) Animal caps pre-treated with Noggin mRNA (100 pg) and either control MO or FoxM1-MO (36 ng) were cultured and analyzed as in C. (E) Animal caps pre-treated or not with Noggin mRNA (100 pg) were incubated for the indicated times and analyzed by RT-PCR. Whereas FoxM1 expression in control animal caps decreased after 2 hours of incubation, that in Noggin-treated animal caps remained constant until 12 hours of incubation, indicating that the induction of FoxM1 expression by Noggin began after 2 hours of incubation. Note that Sox2 began to be expressed coincidently with FoxM1, whereas expression of N-CAM and N-tubulin began after FoxM1, in Noggin-treated animal caps. Image published in: Ueno H et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 16 neuroectoderm neurog2.L atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin-related-1, neurogenin2, ngn-2, ngn2, ngnr, ngnr 1a, Ngnr-1, Ngnr1, X-ngnr-1, xngn2, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 14 neuron ncam1.S n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Throughout NF stage 16 neural plate myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 14 mesoderm neurog2.S atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin-related-1, neurogenin2, ngn-2, ngn2, ngnr, ngnr 1a, Ngnr-1, Ngnr1, X-ngnr-1, xngn2, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 14 neuron Image source: Published Larger Image Printer Friendly View

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