Xenbase Image ID: 24127

Fig. 3. Expression profiles of Xenopus laevis USF genes during embryonic development. WMISH analysis with digoxigenin-labeled probes was performed to determine the spatial expression patterns of USF genes during development. Stained embryos are shown at six stages as labeled in the figure. In the images of late blastula- (stages 9–9.5) and gastrula-stage (stages 10.5–11) embryos, arrowheads indicate the dorsal lip positions (blastula, initial dorsal lip pigment; gastrula, fine dorsal lip). A higher-magnification view of the gray rectangular region in panel O is shown in panel O′ [black dashed line: mesoderm (meso), white dashed line: border of epithelial layer (e.l.) and sensorial layer (s.l.); bc: blastocoel]. White dashed lines in panels E and P indicate the positions of the cuts made to give the cross-sections in panels F and Q. Panels F and Q are WMISH images of cut embryos at stages 14–15 (nc, notochord; e.l., epithelial layer of neuroectoderm; s.l., sensorial layer of neuroectoderm; s.m., somitic mesoderm; en, endoderm). The borders of the three germ layers are indicated at the left side of the embryo as dashed lines. Different views of stage 19 and 25 embryos are shown (H, J, S and U dorsal; G, I, R and T, lateral). Panels K and V are lateral views of late tailbud-stage embryos (stage 33). The dashed line with arrowheads indicates the somite stained with the USF1 probe (panel K). Arrowhead (white) indicates the lateral plate mesoderm stained with the USF2 probe (panel V). Small lines (a–d) in panels K and V point to where the transverse sections of the stage 33 WMISH sample (Fig. 5) were taken. Panels K′ and V′ are higher-magnification views of the gray boxes in panels K and V (br, brain; ov, otic vesicle; ey, eye; ba, branchial arches). Inset in V is a highly contrasted view of the area indicated by dashed box in panel V (pronephros). Image published in: Fujimi TJ and Aruga J (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue usf1.S bhlhb11, fchl, fchl1, hyplip1, LOC108699494, mltf, mltfi, uef X. laevis Throughout NF stage 9 blastocoel roof marginal zone usf1.S bhlhb11, fchl, fchl1, hyplip1, LOC108699494, mltf, mltfi, uef X. laevis Sometime during NF stage 14 to NF stage 19 neural plate neuroectoderm notochord presomitic mesoderm neuroectoderm inner layer cranial neural crest sensorial layer of neurectoderm usf1.S bhlhb11, fchl, fchl1, hyplip1, LOC108699494, mltf, mltfi, uef X. laevis Sometime during NF stage 25 to NF stage 33 and 34 otic vesicle eye somite pharyngeal arch cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest presomitic mesoderm paraxial mesoderm brain neural tube head region usf2.S bhlhb12, usf2-a, usf2-b X. laevis Sometime during NF stage 9 to NF stage 10 blastocoel roof marginal zone mesoderm usf2.S bhlhb12, usf2-a, usf2-b X. laevis Sometime during NF stage 10.5 to NF stage 11 endoderm mesoderm ectoderm blastocoel roof sensorial layer of neurectoderm epidermis outer layer usf2.S bhlhb12, usf2-a, usf2-b X. laevis Sometime during NF stage 14 to NF stage 19 neural plate neural crest neuroectoderm inner layer sensorial layer of neurectoderm pre-chordal neural plate pre-chordal neural plate border usf2.S bhlhb12, usf2-a, usf2-b X. laevis Throughout NF stage 25 otic vesicle eye lateral plate mesoderm pronephric kidney somite neural tube tail tip pronephric duct glomus head region brain cardiac neural crest anterior branchial crest posterior branchial crest mandibular crest hyoid crest tail bud pronephric mesenchyme usf2.S bhlhb12, usf2-a, usf2-b X. laevis Throughout NF stage 33 and 34 otic vesicle eye branchial arch pronephric kidney pronephric duct head region brain neural tube tail tip Image source: Published Larger Image Printer Friendly View

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