Xenbase Image ID: 24279

Fig. 3. Overexpression of FoxD3 promotes neural crest differentiation as well as neuronal differentiation in vivo and in vitro. (A-D) FoxD3 mRNA (100pg) was injected into the left animal blastomeres at the 8-cell stage. Embryos were harvested at stage 23 and subjected to whole-mount in situ hybridization with (A) Slug, (B) FoxD3, (C) Ets-1, and (D) Sox2 probes. Ectopic expression is shown by arrows. (E-G) Overexpression of FoxD3 in animal cap explants. Animal caps were prepared from embryos injected with (E) control mRNA (25 pg) or (F) FoxD3 mRNA (25 pg), and harvested when siblings (E inset) reached stage 40. (G) RT-PCR analysis. Animal caps injected with control or FoxD3 mRNA were harvested at stage 17 equivalent. (H) Overexpression of XBF2 in animal cap explants. Animal caps injected with control (25 pg) or XBF2 (25 pg) mRNA were analyzed as in G. Image published in: Sasai N et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 Xenopus Throughout NF stage 23   foxd3 fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 Xenopus Throughout NF stage 23   snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 Xenopus Throughout NF stage 23   ets1 c-ets1b, ets-1, ewsr2, xe1-a Xenopus Throughout NF stage 23   Image source: Published Larger Image Printer Friendly View

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