Xenbase Image ID: 24281

Fig. 5. FoxD3 is required for neural crest development both in vivo and in animal caps. (A-C) Injection of FoxD3delN mRNA (50 pg) into two right blastomeres at the 8-cell stage suppressed Slug (A), and endogenous FoxD3 (detected with 3′UTR probe) (B). Sox2 was induced in the expected neural crest region (shown by an arrow in C) at stage 16. (D-O) Co-injection of FoxD3delN and Slug rescues the expression of neural crest markers. Injection of Slug mRNA (100 pg) into two right blastomeres at 8-cell stage moderately expands the expression of endogenous Slug (detected with 3′UTR probe) (D), FoxD3 (G) and Twist (J), but not Sox2 (M). Injection of FoxD3delN suppressed expression of the neural crest markers (E,H,K), while co-injection of FoxD3delN and Slug rescued their expression (F,I,L). Expansion of Sox2 caused by FoxD3delN was suppressed by coinjecting Slug (N,O). (P) Animal caps were prepared from embryos injected with Slug (50 pg) + Wnt3a (50 pg; lane 3), Slug (50 pg) + Wnt3a (50 pg) + FoxD3delN (100 pg; lane 4), and Slug (50 pg) + Wnt3a (50 pg) + XBF2delN (100 pg; lane 5) mRNAs. They were harvested at stage17 equivalent and analyzed by RT-PCR. Image published in: Sasai N et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 Xenopus Throughout NF stage 16 neural crest neuroectoderm dorsal anterior foxd3 fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 Xenopus Throughout NF stage 16 neural crest dorsal anterior sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 Xenopus Throughout NF stage 16 neural plate dorsal neuroectoderm Image source: Published Larger Image Printer Friendly View

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