Xenbase Image ID: 24284

Fig. 1. Ectopic expression of Xmsx-1 in the anterior endoderm suppresses head structure formation. Xenopus embryos were either uninjected (A and C, lanes 1, 4, 5) or injected with 500 pg of Xmsx-1 (B and C, lane 2) or 200 pg of BMP-4 (C, lane 3) mRNA in the submarginal zone of the two dorsal blastomeres at the 4-cell stage. (A,B) The embryos were cultured until stage 40 to observe the phenotypes. (C) RT-PCR analysis for the early anterior endomesodermal markers. The dorso-vegetal quarters of the embryos were dissected at stage 10.25 (lanes 1-3) and then subjected to RT-PCR analysis for the indicated markers. Lanes 4 and 5, whole embryo control with (lane 4) or without (lane 5) the RT step. Image published in: Yamamoto TS et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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