Xenbase Image ID: 24293

Fig. 1. Inhibition of XNF-ATc3 affects neural tube closure. (A) Embryos were either injected in the prospective ectoderm at the one-cell stage with 2 ng DN XNF-ATc3 or treated with 4 mM cyclosporin A (CsA) at stage 10 for 1 hour. Both sets were analyzed for Eng, Krox20, Six1, Pax3, Sox2 and HoxB9 expression at late neurula stages (19-21) by in situ hybridization. Neural tube closure defects were seen at concentrations as low as 250 pg DN XNF-ATc3. White arrowheads indicate expansions in the neural marker expression. (B) WT XNF-ATc3 rescues neural CE defects caused by CsA treatment. The graph represents three different experiments (n=731), which were normalized by the percentage of neural CE defects in embryos that were incubated in 400 μM CsA starting at the 64-cell stage. Co-injection of 2 ng WT XNF-ATc3(4) lead to a rescue of neural CE defects. Image published in: Borchers A et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue six1 XSix1 Xenopus Sometime during NF stage 19 to NF stage 21 eye primordium anterior neural fold anterior placodal area pax3 cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 Xenopus Sometime during NF stage 19 to NF stage 21 neural plate neural tube posterior neural tube anterior neural tube hatching gland sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 19 to NF stage 21 neuroectoderm neural tube posterior neural tube anterior neural tube egr2 EGR-2, Krox-20, krox20, XKr20, XKrox-20 Xenopus Sometime during NF stage 19 to NF stage 21 rhombomere R3 rhombomere R5 hoxc9-like.L X. laevis Sometime during NF stage 19 to NF stage 21 paraxial mesoderm en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Sometime during NF stage 19 to NF stage 21 presumptive midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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