Xenbase Image ID: 24295

Fig. 3. Activation of XNF-ATc3 generates neural CE defects and anterior truncations. (A) Xenopus embryos were injected at the one-cell stage with 100 pg (middle panels) or 500 pg (right panels) of CA XNF-ATc3. Embryos injected with 100 pg CA XNF-ATc3 resembled control embryos during gastrula stages (first row, vegetal view of stage 11; second row, vegetal view of stage 12.5), but developed neural tube CE defects in 31% of embryos at neurula stages (third row: dorsal view of stage 17). Embryos injected with 500 pg CA XNF-ATc3 exhibited slight defects at stage 12.5 and neural CE defects in 81% of injected embryos. Control embryos developed no neural CE defects. (B) Xenopus embryos were injected at the one-cell stage with 50 pg CA XNF-ATc3 and analyzed for Krox20, Eng, Pax3, Sox2 and HoxB9 expression at the neurula stage. For XAG-1 in situ hybridization embryos were injected with 250 pg CA XNF-ATc3 and 100 pg lacZ (red) for lineage tracing. Image published in: Borchers A et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue pax3 cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 Xenopus Throughout NF stage 20 neural tube posterior neural tube anterior neural tube hatching gland sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 Xenopus Throughout NF stage 20 neural plate neuroectoderm neural tube trigeminal placode egr2 EGR-2, Krox-20, krox20, XKr20, XKrox-20 Xenopus Throughout NF stage 20 cranial neural crest rhombomere R3 rhombomere R5 hoxb9 hox 2.5, xhoxb9, XlHbox-6, XlHbox6 Xenopus Throughout NF stage 20 paraxial mesoderm posterior neural tube ag1 ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 Xenopus Throughout NF stage 20 cement gland primordium en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 20 presumptive midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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